KRIBIOLISA™ D-Arg8-vasopressin ELISA

SKU: KBI5006 Category:

47,000.00

Immunoassay for quantitative estimation of D-Arg8 Vasopressin in human serum, plasma and tissue extracts.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Vasopressin, also named antidiuretic hormone (ADH), arginine vasopressin (AVP) or argipressin, is a hormone synthesized as a peptide prohormone in neurons in the hypothalamus, and is converted to AVP. AVP has two primary functions; it increases the amount of solute-free water reabsorbed back into the circulation from the filtrate in the kidney tubules of the nephrons. Secondly, AVP constricts arterioles, which increases peripheral vascular resistance and raises arterial blood pressure. (D-Arg8)-Vasopressin is an analogue of Arg8-Vasopressin. It works as an agonist at the V2 vasopressin renal receptor (V2R).

Intended Use:
The D-Arg8 Vasopressin ELISA is used for estimation of D-Arg8 Vasopressin in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes.

Principle:
The D-Arg8 Vasopressin ELISA is a competitive immunoassay for the determination of D-Arg8 Vasopressin. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific D-Arg8 Vasopressin bound.










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Additional information

Species

Human

Sample Type

Human Serum, plasma and tissue extracts

Calibration Range

0.1 ng/ml – 1000 ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Diabetes / Metabolism

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

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