Description
Background:
Darbepoetin alfa is a re-engineered form of erythropoietin that binds to the erythropoietin receptor on erythroid progenitor cells, stimulating RBC production and differentiation. It stimulates erythropoiesis (increases red blood cell levels) by the same mechanism as rHuEpo (binding and activating the Epo receptor) and is used to treat anemia, commonly associated with chronic kidney failure and cancer chemotherapy.
Intended Use:
For Estimation of Darbepoetin (ARANESP) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique Standards or samples are pipetted into microwells pre-coated with Anti-Darbepoetin antibody and Darbepoetin present in the sample and standards are bound by Anti-Darbepoetin antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Darbepoetin present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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