KRIBIOLISA™ Dirucotide (MBP8298) ELISA

SKU: KBI5011 Category:

47,000.00

Immunoassay for quantitative estimation of Dirucotide in human serum, plasma and tissue extracts.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Dirucotide (MBP8298) was developed by two research scientists (Dr. Kenneth G. Warren & Ingrid Catz at the University of Alberta) for the treatment of Multiple Sclerosis (MS). Dirucotide is a synthetic peptide that consists of 17 amino acids linked in a sequence identical to that of a portion of human myelin basic protein (MBP). The drug is exclusively licensed by BioMS Medical Corp., a Canadian-based biotechnology company. BioMS Medical received clearance from the Food & Drug Administration (FDA) to initiate a phase III clinical trial, named MAESTRO-03, for secondary progressive MS patients in January 2007. An additional Phase III clinical trial for dirucotide, MAESTRO-01, is being undertaken in Canada and Europe. In September 2008, the drug was granted FDA fast-track for approval. On July 2009, a statement was released, stating & quot;BioMS Medical Corp. today announced that Dirucotide did not meet the primary endpoint of delaying disease progression, as measured by the Expanded Disability Status Scale (EDSS), during the two-year MAESTRO01 Phase III trial in patients with secondary progressive multiple sclerosis (SPMS). In addition, there were no statistically significant differences between Dirucotide and placebo on the secondary endpoints of the study& quot;, this means that the MAESTRO-02 and MAESTRO-03 trials are discontinued.

Intended Use:
The Dirucotide ELISA is used for estimation of Dirucotide in serum, plasma, or tissue extracts in pharmacokinetics, peptide delivery study and other purposes.

Principle:
The Dirucotide ELISA is a competitive immunoassay for the determination of Dirucotide. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Dirucotide bound.










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Additional information

Species

Human

Sample Type

Precoated plates using monoclonal antibodies; Sample type – Serum, Plasma, or Tissue extracts

Calibration Range

0.1-1000 ng/ml

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Neuro-Degerative Diseases

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.