Description
Background:
Etaracizumab, also known as MEDI-522, trade name Abegrin, is a humanized monoclonal antibody which is being investigated for the treatment of metastatic melanoma, prostate cancer,ovarian cancer and various other types of cancer.It is manufactured by MedImmune. It is an enhanced iteration of Vitaxin, also known as MEDI-523.
Intended Use:
For Estimation of Etaracizumab (ABEGRIN) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Etaracizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Etaracizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Etaracizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Etaracizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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