FastNuclease Endonuclease Serratia Marcescens

Krishgen?s FastNuclease Enzyme (also referred to as Nuclease, Serratia marcescens’ extracellular endonuclease with CAS No.: 9025-65-4) is a recombined Serratia marcescens? extracellular endonuclease expressed in Escherichia coli (E. coli). It has a purity of > 95%, as determined by SDS-PAGE and endotoxin levels < 0.22 EU/1000 units as determined by the LAL method. FastNuclease has a predicted N terminal of three isoforms with different N terminal from the compound?Sm1 (22D-266N), Sm2 (23T-266N) and Sm3 (25E-266N), the activity analysis shows that they were functionally equivalent. FastNuclease is lyophilized from sterile 50 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 5 % trehalose, 5 % mannitol, and 0.01 % Triton? (Triton? is a trademark of Union Carbide, USA). Follow the instructions on the vial. Centrifuge the vial at 4? before opening to recover the entire contents. Please contact us for any concerns or special requirements. Major Applications of FastNuclease 1. Cell Lysis and Lysate Clarification- Removes nucleic acids from lysates to reduce viscosity and facilitate protein extraction 2. Protein Purification - Eliminates DNA/RNA contamination from protein prep workflows (e.g., chromatography) 3. Biopharmaceutical Production - Used in recombinant protein, viral vector, and monoclonal antibody manufacturing to reduce host cell DNA to acceptable regulatory limits (e.g., ?10 ng/dose per WHO/FDA). The residual FastNuclease can be estimated using our Krishgen FastNuclease Endonuclease Serratia Marcescens ELISA (SKU: KBBA36) 4. Downstream Purification of Vaccines and Gene Therapies - Clears nucleic acid contaminants from AAV, lentiviral, and adenoviral vector preps 5. Enzyme or Antibody Prep for ELISA / Western Blot - Reduces background by eliminating nucleic acid-bound proteins 6. Sample Prep for Electrophoresis / Chromatography - Prevents smearing and viscosity during SDS-PAGE, SEC, or HPLC 7. Flow Cytometry Sample Prep - Helps reduce clumping of nuclei-rich samples for better cell suspension 8. Cell-Free Expression Systems - Used to clear residual template DNA/RNA from transcription-translation reactions