Glycobiology
Glycans Decide Whether A Biologic Works.
Glycosylation is a critical quality attribute – it shapes potency, half-life, immunogenicity and biosimilar comparability. Krishgen gives you the enzymes, dyes and analytical tools to release, label and profile every glycan in your molecule, from discovery bench to GMP release.
A glycan profile is a fingerprint your regulator reads.
For monoclonal antibodies, fusion proteins, biosimilars and emerging cell & gene therapy vectors, the attached sugar structures are not a detail — they govern how the molecule behaves in the body and whether it clears review. Four reasons it sits on every characterisation plan.
Featured Products
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Price range: Rs 90,300.00 through Rs 249,500.00 Add to Cart This product has multiple variants. The options may be chosen on the product page -
Price range: Rs 90,300.00 through Rs 249,500.00 Add to Cart This product has multiple variants. The options may be chosen on the product page -
Price range: Rs 90,300.00 through Rs 249,500.00 Add to Cart This product has multiple variants. The options may be chosen on the product page -
Price range: Rs 26,000.00 through Rs 47,700.00 Add to Cart This product has multiple variants. The options may be chosen on the product page
Effector Function
Core fucosylation and terminal galactosylation tune ADCC and CDC. Small shifts in the Fc glycan change how strongly an antibody recruits the immune system.
Immunogenicity
Non-human glycans such as α-Gal and NGNA can trigger immune responses. You need to detect and quantify them before they reach a patient.
Half-life & Clearance
Sialylation and exposed mannose drive serum half-life through receptor-mediated clearance, directly shaping dose and pharmacokinetics.
Biosimilar Comparability
A biosimilar must match the reference glycan map lot after lot. Released-glycan profiling is the evidence regulators expect in the dossier.
Our Own Recombinant Glyco-enzymes & Dyes
Designed, validated and produced under ISO 13485 at Krishgen — recombinant, lot-consistent and priced for Indian biopharma. Available in liquid and lyophilised formats with full documentation.
Krishzyme™ SWIFT PNGase F, recombinant (SWIFT PNGase F)
A mutant recombinant PNGase F (cloned from Flavobacterium meningosepticum, expressed in E. coli) engineered for fast, complete release of high-mannose, hybrid and complex N-glycans.
Krishzyme™ β-N-Acetylhexosaminidase (KPGF-007)
A 55 kDa recombinant exoglycosidase from Streptomyces plicatus for removing terminal GlcNAc / O-GlcNAc residues during sequential glycan sequencing.
Krishzyme™ 2-AB Labelling Dye (KPGF-401)
2-Aminobenzamide fluorescent label for released glycans, validated for HILIC-(U)HPLC, WAX-HPLC and MALDI-MS detection workflows.
KRISHZYME™ Glycosidase & Labelling Range (Full panel)
The complete in-house endo- and exoglycosidase panel plus labelling and clean-up reagents for building a full release-to-profile method. Bulk and OEM formats on request.
We Supply Every Step
Glycan analysis is a genuine sequence — and each stage maps to a defined family of Krishgen reagents and tools. Build a complete released-glycan method without juggling four vendors.
The Glycan Characterisation Workflow
Cleave N- and O-linked glycans from the protein backbone with recombinant glycosidases under native or denaturing conditions.
Trim defined residues with exoglycosidase panels to confirm linkage and sequence, then clean up for downstream analysis.
Tag released glycans with fluorophores for sensitive detection by HILIC-UHPLC, WAX-HPLC and MALDI-MS.
Separate, quantify and identify each glycan against standards and controls for release-grade quality data.
Deglycosylation Enzymes:
Deglycosylation Kits:
| KPGF-100 | Krishzyme Protein Deglycoslyation-O Kit | 1 kit |
| KPGF-200 | Krishzyme Protein Deglycoslyation-NO Kit | 1 kit |
| KPGF-300 | Krishzyme Protein Deglycoslyation-NO2 Kit | 1 kit |
Labeling:
| KPGF-020 | 2 AB Labellng Kit | 25 rxns |
| KPGF-025 | 2-AA Labelling Kit | 25 rxns |
| KPGF-030 | Post Deglycosylation Clean up Cartridges | 1 set of 10 cartridges |
| KPGF-040 | Post Labelling Clean up Cartridges | 1 set of 10 cartridges |
| KPGF-050 | Manual Cleanup Station Module | 1 unit set |
All-in-One Deglycosylation-Labelling Kits
| KPGF-400-1 | Krishzyme KriGly Protein Deglycoslyation-N Kit (column method) | 1 kit / 5 rxns |
| KPGF-400-2 | Krishzyme KriGly Protein Deglycoslyation-N Kit (plate method) | 1 kit / 24 rxns |
| KPGF-400-3 | Krishzyme KriGly Protein Deglycoslyation-N Kit (plate method) | 1 kit / 96 rxns |
| KPGF-500-1 | Krishzyme Swift Protein Deglycoslyation-N Kit (Magnetic Bead based) | 1 kit / 5 rxns |
| KPGF-500-2 | Krishzyme Swift Protein Deglycoslyation-N Kit (Magnetic Bead based) | 1 kit / 24 rxns |
| KPGF-500-3 | Krishzyme Swift Protein Deglycoslyation-N Kit (Magnetic Bead based) | 1 kit / 96 rxns |
