GENLISA Avian Melatonin (MT) ELISA

SKU: KLA003

Rs 34,000.00

Enzyme Immunoassay for the estimation of Avian Melatonin (MT) ELISA in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.

Assay Range: 15.63 – 1000 pg/ml | Sensitivity: 4.94 pg/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

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This GENLISA Avian Melatonin (MT) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Principle Of Assay: This ELISA is a competitive inhibition assay (also called a competitive ELISA) is a powerful assay format used to detect small antigens or haptens that cannot be simultaneously bound by two antibodies (as in sandwich ELISA).

In competitive ELISA, the sample / standard antigen competes with a labeled antigen for a limited amount of capture monoclonal antibody. The more antigen present in the sample, the less labeled antigen is bound, resulting in a lower signal. So, signal intensity is inversely proportional to the concentration of the target antigen.

Disclaimer: The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

Validated against seven points for a GOLD RING Standard Quality ELISA – the benchmark sign for Krishgen Quality. The This GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, tissue homogenates and other biological fluids. The kit uses monoclonal antibodies as capture and labeled for competition to ensure a high degree of sensitivity and specificity in the estimation of the analyte.

Additional information

Pack Type

1 x 96 wells, break-apart

Species Reactivity

Avian

Sample Type

Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only., plasma, Serum

Short Name

Avian Melatonin (MT) ELISA

Assay Range

15.63 – 1000 pg/ml

Standards Available

0; 15.625; 31.25; 62.5; 125; 250; 500; 1000 pg/ml

Calibration Curve

8 point; standards available

Sensitivity

4.94 pg/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Melatonin (MT)

Principle Of Assay

Competitive Inhibition

Detection Method

Absorbance measured at 450nm, Colorimetric

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results

ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Melatonin (MT)

Research Area

Metabolic Pathway; Tumor Immunity; Neuroscience; Hormone Metabolism

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.

Documents

Validation of KBH0321 Human Terminal Complement Complex C5b 9 ELISA version 1.0