GENLISA Human 20-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA

SKU: KBH21137

Rs 30,000.00

Enzyme Immunoassay for the estimation of 20-Hydroxyeicosatetraenoic Acid (12-HETE) in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.

Assay Range: 0 – 1000 ng/ml* (May vary. Please confirm) | Sensitivity: 30 ng/ml* (May vary. Please confirm)

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

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All our kits are manufactured under ISO 13485.

The GENLISA Human 20-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of 20-Hydroxyeicosatetraenoic Acid (12-HETE)

Additional information

Pack Type

1 x 96 wells, break-apart

Species Reactivity

Human

Sample Type

Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only., plasma, Serum

Assay Range

0 – 1000 ng/ml* (May vary. Please confirm)

Standards Available

* request IFU

Sensitivity

30 ng/ml* (May vary. Please confirm)

Principle Of Assay

Competitive Inhibition

Detection Method

Absorbance measured at 450nm, Colorimetric

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

20-Hydroxyeicosatetraenoic Acid (12-HETE)

Research Area

Immune molecule

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.