Description
Introduction:
Rapid and accurate laboratory diagnosis of rabies in humans is essential for timely administration of post exposure prophylaxis. Within a few hours, a diagnostic laboratory can determine whether or not a person has been infected with rabies.
Intended Use:
Human Anti-Rabies Virus Glycoprotein IgG ELISA is specifically designed for the accurate quantitation of antibodies that have strong affinity to epitope (antigenic site) to Rabies Glycoprotein in human serum. It has proven to be an effective assay for detection of rabies.
Principle:
Microtiter wells coated with Rabies Virus are exposed to test specimens, which may contain Rabies IgG reactive antibodies. After an incubation period, unbound components in the test sample are washed away. Specific bound Rabies Glycoprotein IgG complex reacts with anti-human IgG conjugated with HRP during a second incubation period. Following a second wash cycle, specific bound enzyme conjugate is detected by reaction with the substrate solution, TMB. The assay is measured for absorbance to indicate the level of Anti- Rabies Virus glycoprotein IgG present in a sample. The assay only screens for the subtypes and is not able to distinguish between them.
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