Description
Introduction:
The influenza infection is an acute feverish virus infection of the respiratory tract. The infection mostly results from a droplet infection and appears as an epidemic or pandemy. The virus and its toxin can lead to a serious inflammation of the bronchial mucosa and a damage of the vessels. After incubation time of 1 to 3 days the symptoms appear suddenly: Followed by a fast raise of temperature, often accompanied by shivering, the leading symptom of catarrhal inflammation appears, contributing to the clinical course of painful dry cough, tracheitis, laryngitis and frequently a rhinitis and conjunctivitis.
Intended Use:
GENLISA? Human Influenza Viruses IgG ELISA is used as an analytical tool for quantitative determination of Influenza Viruses IgG in human serum, plasma and cell culture supernatant.
Principle:
This is sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Influenza Viruses IgG in samples. Addition of standard or sample to microtiter well which is pre-coated with Human Influenza Viruses IgG monoclonal antibody and addition of biotin labeled Human Influenza Viruses IgG antibody, followed by addition of HRP conjugate to form immune complex. Unbound HRP conjugate will get removed by washing step after incubation. Then addition of Substrate A and B, develops blue color during incubation period and reaction will get stop after addition of stop solution with development of yellow color. The concentration of the Human Influenza Viruses IgG of sample is directly proportional to the yellow color developed in well and will be positively correlated.
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