GENLISA™ Human Interferon Gamma (IFNgamma / IFNg) ELISA

SKU: KB1053 Category:

36,000.00

Enzyme Immunoassay for the estimation of Human Interferon Gamma (IFNgamma / IFNg) in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells





Description

Interferon gamma (IFN-) is a dimerized soluble cytokine that is the only member of the type II class of interferons. It is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils.

ELISA Features include:- Ready to use protocol- Standardisation and High Reproducibility- Lot to Lot Consistency- Accuracy and PrecisionValidated against seven points for a Gold Ring Standard Quality ELISA – the benchmark sign for Krishgen quality. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma, biological fluids and cell culture supernatant.

Background:
Interferon-gamma (IFN-gamma) is a cytokine that plays a crucial role in the immune system’s response to infections, particularly in the context of cellular immunity. It is produced by activated T lymphocytes and natural killer (NK) cells in response to antigenic stimulation. IFN-gamma has diverse functions, including enhancing the activity of immune cells, promoting antigen presentation, and modulating the immune response. In clinical settings, IFN-gamma is used as a diagnostic tool, particularly in assessing cell-mediated immunity. The Quantiferon-TB Gold test, for example, utilizes IFN-gamma release assays to detect the presence of Mycobacterium tuberculosis infection by measuring the IFN-gamma released from T cells exposed to specific tuberculosis antigens. Additionally, IFN-gamma is explored in immunotherapy for various diseases, including certain cancers and autoimmune disorders, where it can exert antitumor or immunomodulatory effects. Its versatile applications in both diagnostics and therapeutics make IFN-gamma a key player in understanding and harnessing the immune system’s capabilities.
The GENLISA Human Interferon Gamma (IFNgamma / IFNg) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Interferon Gamma (IFNgamma / IFNg);









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Additional information

Pack Type

1 x 96 wells, break-apart wells

Species Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only.

Assay Range

15.63 – 1000 pg/ml

Sensitivity

5.9 pg/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Interferon Gamma (IFNgamma / IFNg)

Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Quality Certification

Validated as per KRISHGEN's Quality Program. GOLD PLUS (GOLD+) validation done. The kit is calibrated against NIBSC calibrator.

KRISHGEN Mandate

High Performance Assays at Affordable Prices

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.

ELISA Type

Sandwich ELISA, Double Antibody

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Interferon Gamma (IFNgamma / IFNg);

Research Area

Cytokine;Tumor immunity;Infection immunity;

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

Citations