GENLISA™ Monkey Interleukin 4 (IL4) ELISA

SKU: KLW0032 Category:

14,000.00 28,000.00

GST Applicable
Enzyme Immunoassay for the quantitative determination Monkey Interleukin 4 (IL4) ELISA in monkey serum, plasma, tissues and other biological samples.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

The GENLISA Monkey Interleukin 4 (IL4) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a “GOLD RING” Standard Quality ELISA-the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies-capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Interleukin 4 (IL4)









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Additional information

Pack Type

1 x 96 wells, break – apart wells

Species

Monkey

Sample Type

serum, plasma, tissue homogenates and other biological fluids.(100ul)

Calibration Range

7.82 – 500 pg/ml

Sensitivity

3.1 pg/ml

Interference

Preparations of the factors listed in the IFU were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Interleukin 4 (IL4)

Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Detection Method

Colorimetric, Absorbance read at 450nm

Conjugate - Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Regulatory Status

For Research Use Only

Shipping Temperature

2 – 8 degrees Celsius

Storage Temperature

Store the product at the temperatures indicated in the datasheet and on the respective reagents.

ELISA Type

Sandwich

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

Minimum 8 – 10 months at time of shipping

Alternate Names / Synonyms

Interleukin; 4; (IL4)

Research Area

Cytokine;Immune molecule;