Description
The Mouse Hepatitis Virus GENLISA? ELISA is used as an analytical tool for qualitative laboratory screening of presence or absence of antibodies against mouse hepatitis virus in the serum of mouse. Mouse hepatitis virus belongs to the family coronaviridae. It causes and epidemic murine illness with high mortality. It is ssRNA virus which is mostly transmitted through respiratory aerosols. Although MHV infection may be asymptomatic, it is highly contagious. The method employs the sandwich enzyme immunoassay technique. Hepatitis specific antigen and control antigen is pre-coated onto microwells in alternating columns. Samples, Calibrator and Controls are pipetted into microwells and Mouse Hepatitis Virus antibodies present in the sample are bound by the antigen. Then, Anti-Mouse IgG:HRP (horseradish peroxidase)-Conjugate is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Hepatitis Virus antibodies present in the sample. The color development is stopped by addition of stop solution. Absorbance is measured at 450 nm.