This GENLISA Rat 5-Methyltetrahydrofolate (5-MTHF) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Principle Of Assay: This ELISA is a competitive immunoassay. Antigen coated on 96 well plates competes with the antigen protein present in the sample or standard respectively to bind with the bioinylated antibody. The wells are washed and a Streptavidin:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of antigen protein present in the standard or samples.
Disclaimer: The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.
Validated against seven points for a GOLD RING Standard Quality ELISA – the benchmark sign for Krishgen Quality. This GENLISA ELISA kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma, tissue homogenates and other biological fluids. The kit uses monoclonal antibodies to ensure a high degree of sensitivity and specificity in the estimation of the analyte.
