GENLISA™ Rat Tyrosine-Protein Kinase Fyn (FYN) ELISA

SKU: KLR5811 Category:

28,000.00

Enzyme Immunoassay for the estimation of Rat Tyrosine – Protein Kinase Fyn (FYN) in rat serum, plasma, tissue homogenate, cell culture supernatant and other biological samples.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

The GENLISA Rat Tyrosine – Protein Kinase Fyn (FYN) ELISA includes features like:
– Ready to use protocol with break – apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a ?GOLD RING? Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Tyrosine – Protein Kinase Fyn (FYN)









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Additional information

Pack Size

1 x 96 wells, break – apart wells

Species

Rat

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only.

Assay Range

0.16 – 10 ng/ml

Sensitivity

0.059 ng/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Betacellulin (BTC)

Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate - Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

ELISA Type

Sandwich ELISA, Double Antibody

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

8 – 10 months at time of shipping

Alternate Names / Synonyms

fyn;FYN;

Research Area

Enzyme & Kinase;

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.