Vitamin D is a fat-soluble hormone essential for bone health and immunity, synthesized via sunlight or ingested via D2/D3, while vitamin D1 is an obsolete term for a, now known, mixture of compounds. Originally Vitamin D1 was thought to be a pure vitamin, but was later found to be a mixture of vitamin D2 and other compounds (lumisterol).
D3 (cholecalciferol) is produced in the skin from sun, while D2 (ergocalciferol) comes from plants. Vitamin D1 (Historical Term): 25-hydroxy vitamin D (25OH Vitamin D) is the primary circulating form of vitamin D used to measure total body status, reflecting intake and sun exposure. It is produced by liver hydroxylation of vitamin D3 or D2.
The GENLISA Vitamin D ELISA kit uses polyclonal antibodies and can be used to estimate Vitamin D and its compounds. It does not accurately estimate the circulating Vitamin D which primarily is the GENLISA Vitamin D2 (Ergocalciferol) ELISA or GENLISA Vitamin D3 (Cholecalciferol). These two Vitamin D metabolites, collectively referred to as 25-hydroxyvitamin D or 25(OH)D, are measured in serum to assess the Vitamin D status. Calcifediol is further hydroxylated by the kidneys and certain immune cells to form calcitriol (1,25-dihydroxycholecalciferol; 1,25(OH)2D), the biologically active form of vitamin D. GENLISA ELISA kits are available for each of these specific markers.
This GENLISA ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. This GENLISA ELISA kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma, tissue homogenates and other biological fluids. The kit uses monoclonal antibodies to ensure a high degree of sensitivity and specificity in the estimation of the analyte.
