KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA

KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA

SKU: KBVH500

Rs 72,000.00

Enzyme Immunoassay for the estimation of KRIBIOLISA CRM197 (Carrier Protein) Quantification in serum, plasma, cell culture supernatant, buffers (including PBS 7.4) and other biological samples.

CRM197 (Cross-Reacting Material 197) is a non-toxic mutant of diphtheria toxin widely used as a carrier protein in glycoconjugate vaccines to enhance immune responses to polysaccharide antigens. Quantifying CRM197 during manufacturing helps monitor process consistency, assess carrier protein content in intermediates and final products, and support in-process control and quality evaluation. This KRIBIOLISA CRM197 ELISA is designed for specific, quantitative measurement of CRM197 in vaccine development and manufacturing workflows. Our ELISA kit has been validated also wih FIna Biosolutions, USA CRM197. Fina Biosolutions offers EcoCRM (CRM197). The founder/CEO, Andrew Lewes is also an adjunct professor at the University of Maryland School of Medicine Center for Vaccine Development, the Uniformed Services University, Dept. of Medicine, and the University of Toledo, Dept of Chemistry.

Assay Range: Qualitative | Sensitivity: 5 ug/ml

 

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

Guaranteed Safe Checkout

All our kits are manufactured under ISO 13485.

The KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a ?GOLD RING? Standard Quality ELISA – the benchmark sign for Krishgen Quality. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of the biomarker – antigen/antibody. This kit has application for Carrier protein monitoring: conjugation development, consistency, comparability.

Additional information

Pack Type

1 x 96 wells, break-apart
Vaccine Development

Conjugate / Toxoid / Polysaccharide Vaccine
Vaccine Technology

Conjugate / Toxoid
Species Reactivity

Human
Sample Type

buffer based solutions (pH7.4) and other biological samples., Cell Culture Supernatant, plasma, Serum
Assay Range

Qualitative
Sensitivity

5 ug/ml
Interference

dilute in sample diluent to minimize matrix effects., extreme pH, High levels of detergents/surfactants, or highly complex adjuvanted matrices may affect recovery and should be evaluated using spike-recovery and dilution linearity. For samples containing high antigen load or polysaccharide-conjugates, organic solvents, strong chaotropes (e.g., urea/guanidine)
Principle Of Assay

Double Antibody, Sandwich ELISA
Detection Method

Absorbance measured at 450nm, Colorimetric
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Validation Guideline

WHO guidance (TRS) for conjugate/toxoid vaccine evaluation; ICH Q2(R2)/Q14 for assay validation (as applicable).
Regulatory Status

For Research Use Only (RUO).
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results
ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

CRM197; Carrier Protein
Research Area

Immune molecule, Vaccine, Virology
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.
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