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The GENLISA Sheep insulin – like growth factors 1 (IGF1) ELISA includes features like:
– Ready to use protocol with break – apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of insulin – like growth factors 1 (IGF1)
If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: kbiinfo@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!
Additional information
| Pack Type | 1 x 96 wells, break – apart wells |
|---|---|
| Species | Sheep |
| Sample Type | Serum, Plasma, Tissue Homogenates and other biological fluids. |
| Assay Range | 1.57 – 100 ng/ml |
| Sensitivity | 0.69 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | This assay recognises both the recombinant and natural protein for insulin – like growth factors 1 (IGF1) |
| Principle Of Assay | This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples. |
| Detection Method | Colorimetric, Absorbance measured at 450nm |
| Conjugate - Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Regulatory Status | Research Use Only. |
| Shipping Temperature | Shipped at Room Temperature or in gel pack to maintain temperature at 2 – 8 degrees Celsius |
| Storage Temperature | if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2 – 8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results |
| ELISA Type | Sandwich ELISA, Double Antibody |
| ELISA Interpretation | Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. |
| Shelf Life Available | Minimum 8 – 10 months at time of shipping |
| Alternate Names / Synonyms | IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor |
| Research Area | Tumor immunity; Endocrinology; |