GENLISA™ Sheep Interleukin 1 Beta (IL-1B / IL1B) ELISA

SKU: KLS0041 Category:

₹28,000.00

GST Applicable
Enzyme Immunoassay for the estimation of Sheep Interleukin 1 Beta (IL1B) ELISA in serum, plasma, tissue homogenates and other biological fluids.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells

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Description

The GENLISA Sheep Interleukin 1 Beta (IL1B) ELISA includes features like:
– Ready to use protocol with break – apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Interleukin 1 Beta (IL1B)









If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: kbiinfo@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!

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Additional information

Pack Type

1 x 96 wells, break – apart wells
Species

Sheep
Sample Type

Serum, Plasma, Tissue Homogenates and other biological fluids.
Assay Range

15.63 – 1000 pg/ml
Sensitivity

5.9 pg/ml
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Specificity

This assay recognises both the recombinant and natural protein for Interleukin 1 Beta (IL1B)
Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate - Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Regulatory Status

Research Use Only.
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2 – 8 degrees Celsius
Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2 – 8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results
ELISA Type

Sandwich ELISA, Double Antibody
ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available

Minimum 8 – 10 months at time of shipping
Alternate Names / Synonyms

IL-1B; IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin
Research Area

Cytokine; Tumor immunity; Infection immunity;

Citations

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