GENLISA Vitamin D3 (25-Hydroxyvitamin D3 / VD3) ELISA

SKU: KLU0311

Rs 34,000.00

Enzyme Immunoassay for the estimation of GENLISA Vitamin D3 (25-Hydroxyvitamin D3 / VD3) ELISA in serum, plasma and tissue homogenates.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells

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Assay Range: 4.94 – 400 ng/ml. | Sensitivity: 2.14 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

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All our kits are manufactured under ISO 13485.

The GENLISA Vitamin D3 (25-Hydroxyvitamin D3 / VD3) ELISA includes apart from multi-species detection, features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. These GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes awhich may be species independent serum, plasma, tissue homogenates and other biological fluids. The kit uses monoclonal antibodies as capture and labeled for competition to ensure a high degree of sensitivity and specificity in the estimation of the analyte.

Additional information

Pack Type

1 x 96 wells, break-apart

Species Reactivity

Multiple Species

Sample Type

plasma and tissue homogenates. For other matrices, please connect with our technical team, Serum

Short Name

Vitamin D3 (25-Hydroxyvitamin D3 / VD3) ELISA

Assay Range

4.94 – 400 ng/ml

Standards Available

0; 6.25; 12.5; 25; 50; 100; 200; 400 ng/ml

Calibration Curve

8 point; standards available

Sensitivity

2.14 ng/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein.

Principle Of Assay

Coated with Antigen, Competitive ELISA

Detection Method

Absorbance measured at 450nm, Colorimetric

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results

ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Vitamin D3; 25-Hydroxyvitamin D3 / VD3

Research Area

Biomarker, Biomolecule, Immunology

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.