Description
Introduction:
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by the placenta. During normal pregnancy the level of this hormone in serum and urine gradually increases up to about the eighth week of pregnancy. hCG has two subunits, namely & alpha; and & beta;. The & alpha;-subunit is similar to the & alpha;-subunit of the anterior pituitary gland glycoprotein hormones, namely, LH, TSH and FSH. In normal pregnancy the increase in hCG starts at about the 5th day after conception and continues to rise until it reaches a maximum at about the eighth week. It is a well known fact that hCG is also a tumour marker which is very important in the diagnosis of choriocarcinoma.
Intended Use:
This kit is intended for determination of Human Chorionic Gonadotropin Hormone (hCG) in human serum.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to hCG are precoated onto microwells. Samples and standards are pipetted into microwells and anti hCG antibodies linked to HRP is added to the wells. hCG present in the sample and standards will bind to the plate and HRP Conjugate simultaneously. Washing is done to remove the unbound samples, standards and HRP conjugate. The ready to use substrate solution (TMB) is added to microwells and color develops directly proportional to the amount of hCG in the standards and sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. Please note that a two-step procedure is included and is to be used for assaying serum from pregnant women.
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