Description
Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be
serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Intended Use:
The GENLISA Human Anti-SARS-CoV-2 (Covid-19) IgM antibody to spike protein qualitative ELISA kit is used as an analytical tool for screening for IgM antibodies to Human SARS-CoV-2 (Covid-19) in human serum and plasma.
Principle:
The method employs sandwich ELISA technique. Human SARS-CoV-2 protein is pre-coated onto microwells.Samples and Controls are pipetted into microwells and IgM Antibodies to human SARS-CoV-2 (Covid-19) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by HRP-conjugated Detection IgM antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of IgM Anti-Human SARS-CoV-2 (Covid-19) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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