GENLISA™ Human Tetraspanin 30 Cluster Of Differentiation 63 (CD63) ELISA

SKU: KBH5055 Category:

28,000.00

GST Applicable
Enzyme Immunoassay for the estimation of Human Tetraspanin 30 Cluster Of Differentiation 63 (CD63) in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

CD63 antigen is a protein that, in humans, is encoded by the CD63 gene. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced.CD63 is a critical player in LMP1 exosomal trafficking and LMP1-mediated enhancement of exosome production and may play further roles in limiting downstream LMP1 signaling.CD63 is a good marker for flow cytometric quantification of in vitro activated basophils for diagnosis of IgE-mediated allergy. The test is commonly designated as basophil activation test (BAT).CD63 has been shown to interact with CD117and CD82.In cell biology, CD63 is often used as a marker for multivesicular bodies, which in some cells are enriched with CD63,[6] as well as for extracellular vesicles released from either the multivesicular body or the plasma membrane

The GENLISA Human Tetraspanin 30 Cluster Of Differentiation 63 (CD63) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Tetraspanin 30 Cluster Of Differentiation 63 (CD63);









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Additional information

Pack Type

1 x 96 wells, break-apart wells

Species Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only.

Assay Range

0.156 – 10 ng/ml

Sensitivity

0.057 ng/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Tetraspanin 30 Cluster Of Differentiation 63 (CD63)

Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Quality Certification

Validated as per KRISHGEN's Quality Program. GOLD PLUS (GOLD+) validation done. The kit is calibrated against NIBSC calibrator.

KRISHGEN Mandate

High Performance Assays at Affordable Prices

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.

ELISA Type

Sandwich ELISA, Double Antibody

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Tetraspanin 30 Cluster Of Differentiation 63 (CD63);

Research Area

Enzyme & Kinase;

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.