GENLISA™ Human Rho-Related Gtp-Binding Protein (RHOB) ELISA

SKU: KBH1609 Category:

36,000.00

Enzyme Immunoassay for the estimation of Human Rho-Related Gtp-Binding Protein Rhob (RHOB) in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Developed to detect and quantify RHOB with exceptional accuracy, this kit empowers researchers to unravel the complexities of cell growth, migration, and cytoskeletal dynamics with unprecedented precision.

Background:
RHOB, a member of the Rho family of small GTPases, plays a pivotal role in regulating diverse cellular processes, including cytoskeletal organization, cell adhesion, and gene expression. As a key modulator of cellular signaling pathways, RHOB influences fundamental biological processes such as cell proliferation, differentiation, and apoptosis. The Human RHOB ELISA Kit provides a reliable and sensitive method for measuring RHOB levels in biological samples, facilitating studies aimed at understanding its physiological functions and pathological implications.

Pathways and Relevance:
The Human RHOB ELISA Kit is indispensable in elucidating pathways associated with cell migration, invasion, and metastasis, making it a valuable tool in cancer research. By quantifying RHOB expression levels, researchers can unravel the mechanisms underlying tumor progression, epithelial-mesenchymal transition (EMT), and metastatic dissemination. Additionally, RHOB is implicated in the regulation of vascular function, immune cell activation, and neuronal development, highlighting its relevance in diverse physiological and pathological contexts.

Current Research and Ongoing Discoveries:
Researchers worldwide are leveraging the Human RHOB ELISA Kit to explore the multifaceted roles of RHOB in health and disease. Ongoing research endeavors include investigating the involvement of RHOB in cancer metastasis, angiogenesis, and therapeutic resistance, with the aim of identifying novel targets for anticancer therapies. Furthermore, researchers are exploring the potential of RHOB as a biomarker for disease prognosis and treatment response in various malignancies. Beyond cancer, studies are unraveling the contributions of RHOB to cardiovascular diseases, neurodegenerative disorders, and immune-mediated pathologies, driving innovation and shaping the landscape of biomedical research.

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Rho-Related Gtp-Binding Protein Rhob (RHOB);









If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: kbiinfo@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!

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Additional information

Pack Type

1 x 96 wells, break-apart wells

Species Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only.

Assay Range

100 – 1600 pg/ml

Sensitivity

85.5 pg/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises both the recombinant and natural protein for Rho-Related Gtp-Binding Protein Rhob (RHOB)

Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Quality Certification

Validated as per KRISHGEN's Quality Program. GOLD PLUS (GOLD+) validation done. The kit is calibrated against NIBSC calibrator.

KRISHGEN Mandate

High Performance Assays at Affordable Prices

Regulatory Status

Research Use Only.

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.

ELISA Type

Sandwich ELISA, Double Antibody

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Rho-Related Gtp-Binding Protein Rhob (RHOB);

Research Area

Immune molecule

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.