Description
Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a competitive ELISA technique.
Intended Use:
The GENLISA Human SARS-CoV-2 (Covid-19) Membrane Protein Antigen ELISA kit is used as an analytical tool for quantitative antigen determination of Human SARS-CoV-2 (Covid-19) Membrane Proteins in human serum and plasma.
Principle:
The method employs sandwich ELISA technique. Standards or Samples are added to the microtiter well which is pre-coated with SARS-CoV-2 Membrane Protein. Plates are washed with wash buffer to remove unbound sample. Anti- Human IgG HRP Conjugate is added to the microplate and incubated to form a complex. After incubation and a washing step TMB Substrate is added. Blue color develops on incubation and the reaction is stopped with a Stop Solution to form a yellow color. The concentration of the SARS-CoV2 Membrane Protein in the samples is directly proportional to the yellow color developed (absorbance) in the wells. Absorbance is measured at 450 nm.
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