GENLISA™ Human Serine / threonine-Protein Kinase B-Raf?BRAF) ELISA

SKU: KBH3906 Category:

₹28,000.00

GST Applicable
Enzyme Immunoassay for the estimation of Human Serine / threonine-Protein Kinase B-Raf?BRAF) in human serum, plasma, cell culture supernatant and other biological samples. Validated for citrated/EDTA plasma only.


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells

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Description

The GENLISA Human Serine / threonine-Protein Kinase B-Raf?BRAF) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Serine / threonine-Protein Kinase B-Raf?BRAF);









If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: kbiinfo@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!

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Additional information

Pack Type

1 x 96 wells, break-apart wells
Species Reactivity

Human
Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only.
Assay Range

0 – 1000 ng/ml* (May vary. Please confirm)
Sensitivity

30 ng/ml* (May vary. Please confirm)
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Specificity

This assay recognises both the recombinant and natural protein for Serine / threonine-Protein Kinase B-Raf?BRAF)
Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Quality Certification

Validated as per KRISHGEN's Quality Program. GOLD PLUS (GOLD+) validation done. The kit is calibrated against NIBSC calibrator.
KRISHGEN Mandate

High Performance Assays at Affordable Prices
Regulatory Status

Research Use Only.
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
ELISA Type

Sandwich ELISA, Double Antibody
ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

Serine / threonine-Protein Kinase B-Raf?BRAF);
Research Area

Immune molecule
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

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