The KRIBIOLISA Carbamazepine (TEGRETOL) ELISA is used as an analytical tool for quantitative estimation of Carbamazepine in human serum and plasma. Carbamazepine, sold under the trade name Tegretol, is an anticonvulsant medication used primarily in the treatment of epilepsy and neuropathic pain. It is used as an adjunctive treatment in schizophrenia along with other medications and as a second-line agent in bipolar disorder. It is on the World Health Organization List of Essential Medicines. In 2018, it was the 204th most commonly prescribed medication in the United States,with more than 2 million prescriptions. The method employs the competitive enzyme immunoassay technique. In the first step, samples and standards are pipetted into microwells. If Carbamazepine is present in the samples or standards, it will form complex with specific antibodies which is pre-coated onto microwells. In the second step, Carbamazepine:HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by a washing step. In the third step, TMB substrate is added to microwells and color develops inversely proportionally to the amount of Carbamazepine present in the samples or standards. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
KRIBIOLISA Carbamazepine (TEGRETOL) ELISA
SKU: KBBA22
Rs 104,500.00
Enzyme Immunoassay for the quantitative estimation of Carbamazepine in human serum and plasma. About the ELISA: – Uses highly specific monoclonal antibodies with low cross reactivity – Recovery rates are between 85 – 115% – Ready to use with a standard protocol with break-apart pre-coated wells – Validated as per US FDA guidelines for Bioassays and well optimized – Shelf life: 1 year
Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells
Additional information
| Target / Pathway | Therapeutic target / pathway (unspecified) |
|---|---|
| Active Molecule / Modality | Biologic Drug / Protein |
| Pack Type | 1 x 96 wells, break-apart |
| Species Reactivity | Homo Sapiens (Human) |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, pleas connect with us to optimize the kit accordingly. |
| Analyte / Drug | TEGRETOL |
| Species / Population Reported for Cmax Value | not available |
| Dose & Regimen Reported | not available |
| Cmax (value + units) | Not reported in label/PI |
| Cmax Source (DOI/URL), where available | not available |
| Sample Dilution | Samples are to be diluted 1:1000 with the Sample Diluent provided in the kit. For more specific dilution instructions, please refer to the most updated IFU enclosed with the kit supplied |
| Assay Range Correlated with Cmax Value, where available | 0 – 5 ng/ml |
| Standards Available | 0; 0.156; 0.3125; 0.625; 1.25; 2.5; 5 ng/ml |
| Calibration Curve | 6/8 point; standards available |
| Sensitivity | 0.1 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells, The standards used are research grade biosimilars referenced to the innovator product. In specfic cases where vailable the kit has also been validated against the innovator / reference product. Refer our detailed validation guide for the same. |
| Detection Method | Colorimetric, Absorbance measured at 450nm |
| Conjugate-Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Shipping Temperature | Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius |
| Storage Temperature | The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity. |
| ELISA Type | Sandwich ELISA, Double Antibody |
| ELISA Interpretation | Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. |
| Shelf Life Available | 8 -10 months at time of shipping |
| Alternate Names / Synonyms | Carbamazepine; TEGRETOL |
| Research Area | Immune Molecule; Cell Biology; Immunology, Tumor |
