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KRIBIOLISA hCG (human Chorionic Gonadotropin) Bioassay ELISA

SKU: KBBA09

Rs 105,000.00

Enzyme Immunoassay for the estimation of hCG (human Chorionic Gonadotropin) Bioassay ELISA in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Availability: 3 – 4 weeks | Pack Size: 1 x 96 wells

 

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Background: This bioassay-format ELISA evaluates the biological activity of hormone or growth factor preparations by measuring receptor-mediated functional responses, supporting potency and comparability testing.

Principle Of Assay: This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Additional information

Pack Type

1 x 96 wells, break-apart
Short Name

hCG (human Chorionic Gonadotropin) Bioassay ELISA
Assay Range

0 – 2000 ng/ml
Standards Available

0; 31.25; 62.5; 125; 250; 500; 1000; 2000 ng/ml
Sensitivity

15 ng/ml
Species Reactivity

Homo Sapiens (Human)
Sample Type

cell culture supernates and other biological fluids. For tear samples, cell lysates, plasma, pleas connect with us to optimize the kit accordingly., please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, Serum, tissue homogenates
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Specificity

The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells
Principle Of Assay

Double Antibody, Sandwich ELISA
Detection Method

Absorbance measured at 450nm, Colorimetric
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component kinetic) and TMB Chromogenic Substrate for color development.
Regulatory Status

Research Use Only. Kit is developed and validated as per ICH/EMA/FDA guidelines. Ask for our detailed validation guide at sales1@krishgen.com
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results
ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

hCG; human Chorionic Gonadotropin; Bioassay
Research Area

Immune molecule; Cell Biology; Immunology, Tumor
Product Catalog

Other
Therapeutic / Biology Type

General
Biological Pathway

hCG?LHCGR (GPCR) ? cAMP/PKA; luteal support
KEGG Target Pathway

hsa04024 (cAMP signaling pathway); hsa04060 (Cytokine-cytokine receptor interaction)
Reactome Pathway ID

R-HSA-2404378 (Hormone signaling); R-HSA-1169091 (Adenylyl cyclase activation)
Active Molecule

hCG (human Chorionic Gonadotropin)
Sample Dilution

Dilute at 1:1000 with the Sample Diluent provided in the kit. Please refer to the IFU enclosed with the kit supplied for specific guidance.
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.
SKU: KBBA09 Category: