KRIBIOLISA™ PD-L1 ELISA

SKU: KBBA52 Category:

10,000.00 28,000.00

GST Applicable
Enzyme Immunoassay for the quantitative determination of PD-L1 in serum, plasma and cell culture supernatant.

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction: Programmed death-ligand 1 also known as cluster of differentiation 274 or B7 homolog 1 is a transmembrane protein that in humans is encoded by the CD274 gene. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM).[6] This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2.









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Additional information

Species

Human

Sample Type

Serum, cell culture supernatant and plasma

Detection Method

Colorimetric

Specificity

80% – 120% to PD-L1

Cross Reactivity

< 0.5% cross-reactivity observed with related biomarker.

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Immune Checkpoints

Alternate Names / Synonyms

CD274, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, CD274 molecule, Programmed cell death ligand 1, hPD-L1

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.