Description
Introduction:
Programmed death-ligand 1 also known as cluster of differentiation 274 or B7 homolog 1 is a Trans membrane protein that in humans is encoded by the CD274 gene. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM).[6] This reduces the proliferation of antigen-specific Tcells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) ? further mediated by a lower regulation of the gene Bcl-2.
Intended Use:
The KRIBIOLISA? PD-L1 Pathway Inhibitor (PD-1) Screening ELISA kit is used for the quantitative estimation of PD-L1 Pathway Inhibitor (PD-1) screening in serum, plasma and biological solutions including pharmaceutical preparations.
Principle:
The PD-L1 Pathway Inhibitor ELISA is a competitive immunoassay for the determination of PD-L1 Pathway Inhibitor. Plates are coated with PDL1 protein. A varying concentration of unlabeled standard antibody or sample and constant concentration of Human PD1/PDCD1 protein:Biotin Conjugate will bind in sequence to the Human PD-L1/B7-H1 protein coated on the microplate. Upon washing, unbound Human PD1/PDCD1 protein:Biotin Conjugate will be removed. Bound Human PD1/PDCD1 protein:Biotin Complex will bind to Streptavidin:HRP to produce a soluble blue colored product after the addition of TMB Substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound PD-L1 Pathway Inhibitor present in the standards or samples.