Background: Tremelimumab (formerly ticilimumab, CP-675,206) is a fully human monoclonal antibody against CTLA-4. It is an immune checkpoint blocker. Cytotoxic T lymphocytes (CTLs) can recognize and destroy cancer cells. However, there is also an inhibitory mechanism (immune checkpoint) that interrupts this destruction. Tremelimumab turns off this inhibitory mechanism and allows CTLs to continue to destroy the cancer cells. Intended Use: For Estimation of Tremilimumab in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization. Principle: The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Tremelimumab antibody and Tremelimumab present in the sample and standards are bound by Anti- Tremelimumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Tremelimumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
KRIBIOLISA Tremilimumab ELISA
SKU: KBI1090
Rs 66,000.00
Enzyme Immunoassay for the estimation of Tremilimumab in serum and plasma. Key features: 1. Developed using highly specific monoclonal anti-idiotypic antibodies in a sandwich assay format 2. Validated as per international bioassay validation guidelines 3. Recovery: 80-120% 4. Precision CV <10%
The below Kit Insert is subject to change with the new lot of kits.
Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells
Additional information
| Target / Pathway | CTLA 4 pathway |
|---|---|
| Active Molecule / Modality | Tremelimumab – IgG2 mAb; blocks CTLA 4 |
| Pack Type | 1 x 96 wells, break-apart |
| Species Reactivity | Homo Sapiens (Human) |
| Sample Type | cell culture supernates and other biological fluids. For tear samples, cell lysates, plasma, pleas connect with us to optimize the kit accordingly., please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, Serum, tissue homogenates |
| Analyte / Drug | Tremilimumab |
| Species Cmax Value | not available |
| Dose & Regimen Reported | not available |
| Cmax (value + units) | Not reported in label/PI |
| Cmax Source (DOI/URL) | not available |
| Sample Dilution | please refer to the most updated IFU enclosed with the kit supplied, Samples are to be diluted 1:1000 with the Sample Diluent provided in the kit. For more specific dilution instructions |
| Assay Range Cmax Value | 0 – 1000 ng/ml |
| Standards Available | 0; 15.625; 31.25; 62.5; 125; 250; 500; 1000 ng/ml |
| Calibration Curve | 6/8 point; standards available |
| Sensitivity | 10 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells |
| Detection Method | Absorbance measured at 450nm, Colorimetric |
| Conjugate-Enzyme Reaction | Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. |
| Shipping Temperature | Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius |
| Storage Temperature | if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results |
| ELISA Type | Double Antibody, Sandwich ELISA |
| ELISA Interpretation | 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results. |
| Shelf Life Available | 8 -10 months at time of shipping |
| Alternate Names / Synonyms | Tremilimumab |
| Research Area | Immune molecule; Cell Biology; Immunology, Tumor |
