Krishzyme Beta Galactosidase releases only beta (1-4)- linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.
It is by far the most common linkage found in N-linked oligosaccharides. The enzyme is active on tetraantennary oligosaccharides as on disaccharides containing beta (1-4)-linked galactose. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.
One unit of Krishzyme Beta Galactosidase is defined as the amount of enzyme required to produce 1 umole of p-nitrophenol (pNP) in 1 minute at 37?C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.