Bsa I , an IIs restriction endonuclease restriction endonuclease, is derived from a recombinant E.coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus. which have the same specificity as native enzymes and reduced star activity. Its recognition sequence and cleavage sites are as
follows:
5???????GGTCTC(N)???????????????3?
3???????CCAGAG(NNNNN)??????5
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 ?g of Internal control DNA in 1 hour at 37?C in a total reaction volume of 50 ?L.
Application includes:
– Restriction Enzyme Digestion
– Fast Cloning