KRISHZYME Hyaluronidase Enzymatic Assay Kit
KRISHZYME Hyaluronidase Enzymatic Assay Kit - Image 2

KRISHZYME Hyaluronidase Enzymatic Assay Kit

SKU: KBBA05

Rs 64,000.00

Enzymatic Assay for Quantification of Hyaluronidase activity in cell culture supernatant and pharmaceutical preparations.

About the kit:
Uses high quality reagents from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
Recovery rates are between 85 ? 115%
Ready to use with a standard protocol with break-apart pre-coated wells
Validated as per US FDA guidelines for Bioassays
Optimized for matrix effects to ensure higher sensitivity.
Shelf life: 1 year

1 in stock

 

Guaranteed Safe Checkout

All our kits are manufactured under ISO 13485.

The KRISHZYME Hyaluronidase Assay kit uses a two-step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.

Hyaluronidase is a family of enzymes that degrade hyaluronic acid by catalyzing the hydrolysis of hyaluronan, a constituent of the interstitial barrier. Hyaluronidase lowers the viscosity of hyaluronan, thereby increasing tissue permeability. It is, therefore, used in medicine in conjunction with other drugs to speed their dispersion and delivery.

Additional information

Pack Type

100 tests / 1 kit
Assay Type

Research / QC Functional
Assay Alignment

EP 2.7.5 & USP compliant
Sample Type

biological preparations and solutions (including buffers), Human blood products
Suggested Assay Range

10 – 1000 U/ml
Principle Of Assay

Hyaluronidase degrades hyaluronic acid; colorimetric/turbidimetric readout
Detection Method

Absorbance., Chromogenic
Substrate Reaction

Uses Chromogenic Substrate for color devlopment
Intended Use

Research / QC Functional. For Research and Testing Use Only. Not for In-Vitro Diagnositc Purposes.
Validation Parameters

accuracy, ICH Q2-style: specificity, linearity, LOD/LOQ (if quantitative), precision (repeatability/intermediate), range, robustness, stability., system suitability
Regulatory Status

For Industrial and QC Use Only. Not for IVD Use.
Shipping Temperature

Room Temperature / 2-8 degrees Celsius
Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, well sealed and store at the indicated temperature.
ELISA Type

Enzyme – Chromogenic Assay
Shelf Life Available

20-24 months at time of shipping
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.
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