Krishzyme Poly (A) Polymerase is expressed by recombinant E.coli. The enzyme does not depend on the template and can catalyze the sequential incorporation of ATP into the 3 end of RNA in the form of AMP. This means by adding a polyadenosine tail to the 3 end of RNA, Poly (A) structure can improve the stability of RNA and improve the translation efficiency of mRNA in eukaryotic cells. Poly (A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3 end of RNA.
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 l volume in 10 minutes at 37 Deg C.
Purity: 95%
Host DNA Residue: 100pg/mg
Host Protein Residue: 50ppm
Endotoxin Residue: 10EU/mg
No DNase and RNase activity
No Protease activity. Sterile, No mycoplasma detected.