Protein deglycosylation is the enzymatic removal of N-linked and O-linked oligosaccharides (glycans) from glycoproteins, critical for studying protein structure, function, and modification. Common tools include PNGase F for N-glycans and O-Glycosidase for O-glycans. This process reduces molecular heterogeneity, facilitating analysis by SDS-PAGE, Western blotting, or mass spectrometry. Applications include Mass Spectrometry: Enables accurate mass determination and identification of glycans; Protein Characterization: Helps determine the presence, type, and impact of glycosylation on protein function and Cellular Studies: Targeted deglycosylation using specialized tools like nanobody-fused enzymes allows for the removal of specific glycan modifications within living cells.
Krishzyme Protein Deglycoslyation-N Kit (plate method)
SKU: KPGF-400-2
Price range: Rs 156,000.00 through Rs 432,000.00
The Krishzyme N-Glycan Kit includes rapid enzymatic digestion using PNGase F, labeling with reagents, purification, and analysis by HPLC/LC-MS. N-glycosylation significantly affects the biological function, half-life, immunogenicity, solubility, thermal stability, protease resistance, and aggregation of proteins. It is a key indicator of batch-to-batch consistency in antibody production. ICH-Q6B, various pharmacopoeias, and regulatory authorities have established quantitative requirements for N-glycan analysis.
Additional information
| Pack Size | 1 kit / 24 rxns, 1 kit / 96 rxns |
|---|---|
| Shipping Temperature | – 20 Deg C, dry ice |
| Storage Temperature | Frost free freezer at -20 Deg Celsius |
| Regulatory Status | For Research Use Only |