Protein deglycosylation is the enzymatic removal of N-linked and O-linked oligosaccharides (glycans) from glycoproteins, critical for studying protein structure, function, and modification. Common tools include PNGase F for N-glycans and O-Glycosidase for O-glycans. This process reduces molecular heterogeneity, facilitating analysis by SDS-PAGE, Western blotting, or mass spectrometry. Applications include Mass Spectrometry: Enables accurate mass determination and identification of glycans; Protein Characterization: Helps determine the presence, type, and impact of glycosylation on protein function and Cellular Studies: Targeted deglycosylation using specialized tools like nanobody-fused enzymes allows for the removal of specific glycan modifications within living cells.
Krishzyme Protein Deglycoslyation-NO2 Kit
SKU: KPGF-300
Rs 70,000.00
Krishzyme Protein Deglycosylation Kit (for N-Linked & Complex O-Linked Glycans) includes the enzymes and reagents which can be used simultaneously for the removal of all N-linked and complex O-linked glycans. One set contains PNGase F (Krishzyme KPGF-003), O-Glycosidase (Krishzyme KPGF-004), alpha2-3,6,8,9 Neuraminidase (Krishzyme KPGF-005), Beta1-4 Galactosidase (Krishzyme KPGF-006) and Beta-N-Acetylhexosaminidase (Krishzyme KPGF-007).
Additional information
| Pack Size | 1 kit |
|---|---|
| Shipping Temperature | – 20 Deg C, dry ice |
| Storage Temperature | Frost free freezer at -20 Deg Celsius |
| Regulatory Status | For Research Use Only |