Protein deglycosylation is the enzymatic removal of N-linked and O-linked oligosaccharides (glycans) from glycoproteins, critical for studying protein structure, function, and modification. Common tools include PNGase F for N-glycans and O-Glycosidase for O-glycans. This process reduces molecular heterogeneity, facilitating analysis by SDS-PAGE, Western blotting, or mass spectrometry. Applications include Mass Spectrometry: Enables accurate mass determination and identification of glycans; Protein Characterization: Helps determine the presence, type, and impact of glycosylation on protein function and Cellular Studies: Targeted deglycosylation using specialized tools like nanobody-fused enzymes allows for the removal of specific glycan modifications within living cells.
Krishzyme Protein Deglycoslyation-O Kit
SKU: KPGF-100
Rs 24,000.00
Krishzyme Protein Deglycosylation-O Kit (for Simple O-linked Glycans) contains O-Glycosidase (Krishzyme KPGF-004) and ?2-3,6,8,9 Neuraminidase (Krishzyme KPGF-005), which can be used simultaneously for the removal of terminal sialic acid residues and core 1 and core 3 O-glycans. One set of this Kit includes 1,200,000 units of O-Glycosidase and 0.6 units of alpha2-3,6,8,9 Neuraminidase.
Additional information
| Pack Size | 1 kit |
|---|---|
| Shipping Temperature | – 20 Deg C, dry ice |
| Storage Temperature | Frost free freezer at -20 Deg Celsius |
| Regulatory Status | For Research Use Only |