The Krishzyme Endoproteinase Asp-N is a specific proteolytic enzyme that cleaves peptide bonds on the N-terminal side of aspartic acid (Asp, D) residues, and sometimes glutamic acid (Glu, E) under certain conditions.
Applications:
Mass spectrometry (LC-MS/MS) – Used for specific fragmentation to identify proteins based on Asp cleavage.
Complements Trypsin Digestion – Trypsin cleaves C-terminal to Lys/Arg; Asp-N gives orthogonal cuts, increasing sequence coverage.
Peptide Mapping – Useful for epitope mapping and structure-function studies
N-terminal Sequencing – Helps in targeted cleavage before Asp residues for Edman degradation or MS workflows