Krishzyme recombinant Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. It is expressed in pichia pastoris and has a moleulcar wiehjgot of 221.11 kDa. It is available in a concentration of > 30 KU / mg,
1mg of recombinant bovine enterokinase can cut at least 1.5g fusion protein. The Krishzyme recombinant Enterokinase has a purity >90% (as determined by SDS-PAGE).
Biological Activity
At 25 ? C, reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U.
Applications:
– Fusion Protein Cleavage,
– Protein Purification
– Protein Digestion