The Krishzyme high-purity TEV Protease, is a reliable enzymatic solution for protein research. Derived from Tobacco Etch Virus (TEV) Nla, and expressed in Ecoli, this recombinant protease exhibits exceptional site specificity, recognizing the seven-amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln?Gly) and cleaving precisely between Gln and Gly. It efficiently removes affinity tags, such as GST or other fusion tags, ensuring precise and effective protein preparation.
Form: Liquid
Buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, 50% glycerol, pH 8.0
Host: E. coli
Molecular Weight: 50 kDa
Purity: >95% (using SDS-PAGE)
Specific Activity: 17 U/ul
Unit Definition: One unit is defined as the amount of enzyme required to cleave >85% of 3 ug of substrate at 30?C, pH 8.0, in 1 hour.
Reaction Conditions: 1 x TEV Protease Buffer (50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, 1 mM DTT, pH 8.0); cleave at 30?C for 1 hour.
Key Advantages:
High Specificity – Krishzyme TEV Protease precisely recognizes the seven-amino acid sequence, ensuring accurate cleavage and avoiding nonspecific cuts.
Broad Reaction Conditions – Retains high activity over a wide range of pH (6.0?8.5) and temperatures (4?30?C), making it versatile for various experimental setups.
Convenient Purification – Features an N-terminal GST tag, allowing easy removal using glutathione affinity chromatography, simplifying workflows.
Applications
Removal of affinity tags (e.g., GST) from fusion proteins
Protein purification and functional studies
Flexible enzymatic cleavage for various experimental conditions