Krishzyme T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage that possesses a strong and specific 5?? 3? RNA polymerase activity. The Krishzyme T7 RNA Polymerase has a high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37?C.
Validation of Quality:
Endonuclease Activity – Incubation of a 40 ?L reaction containing a minimum of 200 U of T7 RNA Polymerase with 4 ug pUC19 DNA for 16 hours at 37? results in no detectable degradation as determined.
Exonuclease Activity – Incubation of a 50 ul reaction containing a minimum of 200 U of T7 RNA Polymerase with 1 ug Hind III digest Gamma DNA for 16 hours at 37? results in no detectable degradation as determined.
Nickase Activity – Incubation of a 50 ul reaction containing a minimum of 200 U of T7 RNA Polymerase with 1 ug pBR322 DNA for 16 hours at 37? results in no detectable degradation as determined.
RNase Activity – Incubation of a 50 ul reaction containing a minimum of 200 U of T7 RNA Polymerase with 1.6 ug MS2 RNA for 4 hours at 37? results in no detectable degradation as determined.