Description
Introduction: The GENLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit. Intended Use: The Monkey mast cell tryptase, MCT GENLISA™ ELISA kit is used as an analytical tool for quantitative determination of Monkey mast cell tryptase, MCT in serum, plasma and other biological samples. Principle: The method employs sandwich ELISA technique. Monkey Mast cell tryptase antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Monkey mast cell tryptase, MCT present in the sample are bound by the antibodies. Biotin labeled MCT antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Monkey mast cell tryptase, MCT in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Background:
Mast cell tryptase (MCT) is an enzyme released by mast cells, a type of immune cell found in various tissues throughout the body. Upon activation, mast cells release granules containing a variety of mediators, including tryptase. MCT functions as a protease, playing a role in immune responses and inflammation. It is involved in the breakdown of proteins, particularly in the extracellular matrix, and can contribute to tissue remodeling. Elevated levels of mast cell tryptase in blood or tissue samples may indicate mast cell activation and are often used as a diagnostic marker for conditions associated with mast cell disorders, such as mastocytosis. Additionally, MCT levels can be measured to assess the severity of allergic reactions and anaphylaxis, as mast cells play a central role in allergic responses and can release tryptase upon exposure to allergens. Understanding mast cell tryptase’s function is crucial for diagnosing and managing conditions related to mast cell activation and immune responses.
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