Description
Background:
Palivizumab is a humanized monoclonal antibody (IgG1k) that binding the (respiratory syncytial virus) RSV envelope fusion protein (RSV F) on the surface of the virus and blocking a critical step in the membrane fusion process. Palivizumab targets the fusion protein of RSV,[8] inhibiting its entry into the cell and thereby preventing infection. Palivizumab was approved for medical use in 1998.
Intended Use:
For Estimation of Palivizumab (SYNAGIS) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Palivizumab antibody and Palivizumab present in the sample and standards are bound by Anti- Palivizumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Palivizumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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