Description
Introduction:
The GENLISA™ ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs an indirect ELISA technique.
Intended Use:
The Porcine Anti-Monkey Pox IgG GENLISA™ ELISA kit is used as an analytical tool for quantitative determination of Porcine Anti-Monkey Pox IgG serum, plasma and other biological samples.
Principle:
The method employs indirect ELISA technique. Antigens are pre-coated onto microwells. Samples and standards are pipetted into microwells and Porcine Anti-Monkey Pox IgG present in the sample are bound by the antigens. Biotin labeled antibody is added and followed by Streptavidin:HRP is pipetted and incubated to form a complex.
After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Porcine Anti-Monkey Pox IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.