KRIBIOLISA™ Sunitinib Malate (SUTENT) ELISA

SKU: KOD1019 Category:

67,500.00

Enzyme Immunoassay for the quantitative determination of Sunitinib in serum, plasma and cell culture supernatant

About the ELISA:
– Uses highly specific monoclonal antibodies with low cross reactivity
– Recovery rates are between 85 – 115%
– Ready to use with a standard protocol with break-apart pre-coated wells
– Validated as per US FDA guidelines for Bioassays and well optimized
– Shelf life: 1 year


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells



Description

Introduction:
Sunitinib (SUTENT) is a medication used to treat cancer. It is a small-molecule, multi-targeted receptor tyrosine kinase (RTK) inhibitor that was approved by the FDA for the treatment of renal cell carcinoma (RCC) and imatinib-resistant gastrointestinal stromal tumor (GIST). Sunitinib Malate is an antineoplastic agent, Flk-1 inhibitor, and ATP-competitive inhibitor of PDGFR beta.

Intended Use:
This kit is used for quantitative estimation of Sunitinib in serum, plasma and cell culture supernatant using a competitive inhibition principle.

Principle:
The KRIBIOLISA Sunitinib ELISA is a competitive inhibition immunoassay for the determination of Sunitinib. The microplate is coated with VEGFA protein. VEGFA protein is a known dimeric glycoprotein which functions as a ligand for the receptor VEGFR2 (Flk1). Samples and standards containing Sunitinib is added to the plate with HRP conjugated VEGFR-2 from the kit simultaneously. The Sunitinib in the samples and standard will inhibit the binding of VEGFR-2 to the microplate. The amount of Sunitinib is inversely proportional to the color developed in the microplate post addition of TMB substrate and stopping the reaction. The absorbance is read at 450 nm.










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Additional information

Species

Human

Sample Type

Serum, plasma and cell culture supernatant

Calibration Range

0 ug/ml – 500 ug/ml

Sensitivity

14 ng/m

Detection Method

Colorimetric

ELISA Type

Direct Sandwich Assay

Regulatory Status

For Research Use Only

Research Area

Cancer

Storage Temperature

Store the unopened product at 2-8 Deg C. Do not use past expiration date.

Validation

The kit uses a biosimilar research grade standard / calibrator. Please refer the IFU for more details.

Disclaimer

Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only.