Speciality Coagulation
Whether you are working with coagulation factors, coagulation inhibitors, or fibrinolysis inhibitors, our extensive range of ELISA provides specific and sensitive results to monitor and quantify coagulation in various human sample matrices.
Hemophilia A
Hemophilia encompasses a group of inherited disorders that alter blood coagulation. Classical haemophilia, also known as haemophilia A, is a hereditary haemorrhagic disorder resulting from a congenital deficit of factor VIII that manifests as protracted and excessive bleeding either spontaneously or secondary to trauma.
Our KRIBIOLISA™ Factor VIII is a chromogenic assay for measuring factor VIII activity. It contains reagents from human origin and is therefore fully sensitive to the factor VIII mimetic Emicizumab (Hemlibra® by Roche) used in haemophilia A treatment.
In case a test kit insensitive to Emicizumab is required, we offer a solution with our KRIBIOLISA™ Factor VIII (bovine reagent component).
In sum, the KRIBIOLISA™ Factor VIII Chromogenic Assay kits allow measuring Emicizumab in treated haemophiliacs (kit with human FX) and evaluating anti-FVIII inhibitor titres in presence of Emicizumab (kit with bovine FX).
Our KRIBIOLISA Emicizumab ELISA (#KBI1297) is a double antibody sandwich assay using Factor IX and Factor X recombinant proteins to capture Emicizumab in human serum and plasma and a biosimilar Emicizumab as standard. The ELISA offers simplicity in testing and directly estimates Emicizumab levels in patient samples.
Hemophilia B
Haemophilia B (also known as Factor IX deficiency) is a type of clotting disorder, much rarer than Haemophilia A (Classic Haemophilia or Factor VIII deficiency).
Our chromogenic assay KRIBIOLISA™ Factor IX Chromogenic Assay and ELISA kit serves to measure factor IX activity in human citrated plasma or factor IX concentrates.
Von Willebrand Factor / ADAMTS-13
Von Willebrand factor (vWF) is glycoprotein crucial to primary hemostasis through platelet and subendothelial collagen adhesion, and the intrinsic coagulation cascade, through factor VIII stabilization.
The von Willbrand factor protects factor VIII against premature proteolytic degradation and secures its transport to where it is needed as co-factor in the course of secondary haemostasis.
For the diagnosis of von Willebrand disease, we offer GENLISA™ VWF ELISA (#KBH1140, Assay Range: 0 – 1,000 ng/ml)
Our GENLISA™ ADAMTS-13 ELISA (#KBH3484) allows determination of ADAMTS-13 antigen with the highest sensitivity.
| Krishgen Human Assays | Krishgen Mouse Assays | Krishgen Rat Assays |
|---|---|---|
| KBH1186 Human von Willebrand Factor (vWF) ELISA | KLM0273 Mouse von Willebrand Factor ELISA | KLR0674 Rat von Willebrand Factor ELISA |
Monitoring of Anticoagulants
- Our monitoring assays cover the entire spectrum of anticoagulants. The anticoagulants used nowadays can be divided into:
- Vitamin K antagonists such as Marcumar®
- Heparins (UFH, LMWH) and heparinoids, e.g. Danaparoid Sodium (Orgaran®) and Fondaparinux (Arixtra®)
- Direct Xa Inhibitors such as Apixaban (Eliquis®), Rivaroxaban (Xarelto®) and Edoxaban (Lixiana®)
- Direct Thrombin Inhibitors (anti-IIa) such as Argatroban (Argatra®), Dabigatran (Pradaxa®), Hirudin and Bivalirudin (Angiox®)
KRISHZYME™ Anti-Factor IIa (KBBA03) / KRISHZYME™ Anti-Factor Xa (KBBA04) Chromogenic Assay Kits
Our simple validated for specific analytes help researchers to use our Factor IIa and Factor Xa chromogenic kits. These kits are validated with NIBSC standards and where claimed against the innovator drugs to make the kits as per the specific protocol suitable for us.
Direct Oral Anticoagulants (DOACs)
In coagulation disorder therapy, the Direct Oral Anticoagulants (DOACs) Apixaban (Eliquis®), Rivaroxaban (Xarelto®), Dabigatran (Pradaxa®) and Edoxaban (Lixiana®) have established themselves increasingly for manufacturers for the QC and end-point release of production lots.
| KBBA03S | KRISHZYME™ Heparin Factor IIa Assay Kit | 100 tests / 1 kit |
| KBBA04S | KRISHZYME™ Heparin Factor Xa Assay Kit | 100 tests / 1 kit |
| KBBA03 | KRISHZYME™ Heparin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04 | KRISHZYME™ Heparin Factor Xa Assay Kit | 200 tests / 1 kit |
| KBBA03B | KRISHZYME™ Bivaluridin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04B | KRISHZYME™ Bivaluridin Factor Xa Assay Kit | 200 tests / 1 kit |
| KBBA03ES | KRISHZYME™ Enoxaparin Factor IIa Assay Kit | 100 tests / 1 kit |
| KBBA04ES | KRISHZYME™ Enoxaparin Factor Xa Assay Kit | 100 tests / 1 kit |
| KBBA03E | KRISHZYME™ Enoxaparin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04E | KRISHZYME™ Enoxaparin Factor Xa Assay Kit | 200 tests / 1 kit |
| KBBA03D | KRISHZYME™ Dalteparin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04D | KRISHZYME™ Dalteparin Factor Xa Assay Kit | 200 tests / 1 kit |
| KBBA03N | KRISHZYME™ Nadroparin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04N | KRISHZYME™ Nadroparin Factor Xa Assay Kit | 200 tests / 1 kit |
| KBBA03T | KRISHZYME™ Tinzaparin Factor IIa Assay Kit | 200 tests / 1 kit |
| KBBA04T | KRISHZYME™ Tinzaparin Factor Xa Assay Kit | 200 tests / 1 kit |
If present in plasma samples, they interfere with a big number of coagulation assays. Our new range of kits are soon being launched to overcome this.
Fibrinolysis
Fibrinolysis is a normal body process. It prevents blood clots that occur naturally from growing and causing problems. Primary fibrinolysis refers to the normal breakdown of clots. Secondary fibrinolysis is the breakdown of blood clots due to a medical disorder, medicine, or other cause. Physiological fibrinolysis means the cleavage of fibrin by the enzyme plasmin. The fibrinolytic system, i.e. the degradation of fibrin, must be in a state of equilibrium with the coagulation system, which provides fibrin deposits.
For the determination of plasminogen activity, we offer our GENLISA™ tPA ELISA (#KBH3707, Assay Range: 0 – 10 ng/ml).
The fibrinolytic system is kept under control by inhibitors (like alpha-2-antiplasmin, PAI-1). For investigating these inhibitors, our following test kits are suited:
GENLISA™ Alpha-2-Antiplasmin ELISA (#KBH0423, Assay Range: 0 – 300 ng/ml)
GENLISA™ PAI-1 ELISA (#KB1099, Assay Range: 0 -800 pg/ml)
| Krishgen Human Assays | Krishgen Mouse Assays | Krishgen Rat Assays |
|---|---|---|
| KB1099 Human PAI-1 ELISA | KLM0088 Mouse PAI- 1 ELISA | KLR0636 Rat PAI-1 ELISA |
| KBH3707 Human Plasminogen Activator Tissue (tPA) ELISA | KLR1758 Rat Tissue-Type Plasminogen Activator ELISA | |
| KBH0909 Human Urokinase Plasminogen Activator (uPA) ELISA | KLM0681 Mouse uPA ELISA | KLR0478 Rat uPA ELISA |
Heparin Induced Thrombocytopenia (HIT-II)
Heparin-Induced Thrombocytopenia (Type II) is a rare and dangerous condition which occurs in 1% to 3% of patients receiving heparin. Thrombocytopenia occurs 5 to 10 days after heparin therapy decreasing the platelet counts by 30 to 50%. Type II HIT primarily affects the venous system but arterial involvement is not uncommon.
GENLISA™ PF4 ELISA specifically detects Platelet Factor 4 in serum and platelet poor plasma. HIT is caused by development of IgG antibodies to heparin-platelet factor 4 (PF4) complexes. This kit helps in estimation of Platelet Factor 4 (#KBH1108 Assay Range: 0 – 100 pg/ml) and helps researchers look at the HIT-II phenomenon in patients.
Other Coagulation Factors
Coagulation Related ELISA available for Other Species:
| Porcine | KLP0198 Porcine Low Molecular Weight Heparin ELISA |
| KLP0436 Porcine Heparin ELISA | |
| KLP0266 Porcine von Willebrand Factor ELISA | |
| KLP0276 Porcine Thrombomodulin ELISA | |
| KLP0264 Porcine Plasminogen Activator Inhibitor 1 ELISA | |
| KLP0217 Porcine Anti-Thrombin Receptor ELISA | |
| KLP0440 Porcine Thrombin-AntiThrombin Complex ELISA | |
| KLP0546 Porcine Coagulation Factor 7 ELISA | |
| KLP0547 Porcine Coagulation Factor 5 ELISA | |
| Cat | KLT0024 Cat von Willebrand Factor ELISA |
| KLT0066 Cat Thrombomodulin ELISA | |
| Rabbit | KLX0364 Rabbit von Willebrand Factor ELISA |
| KLX0245 Rabbit Urokinase-Type Plasminogen Activator ELISA | |
| KLX0362 Rabbit Tissue Factor Pathway Inhibitor ELISA | |
| KLX0361 Rabbit Thrombin-Antithrombin Complex ELISA | |
| Bovine | KLB0115 Bovine Plasminogen Activator Inhibitor 1 ELISA |
| KLB0345 Bovine Plasminogen Activator (Urokinase) ELISA | |
| KLB0195 Bovine Anti-Thrombin 3 ELISA | |
| KLB0199 Bovine Thrombin-Antithrombin Complex ELISA | |
| Canine | KLN0028 Canine Tissue Factor pathway Inhibitor ELISA |
| KLN0022 Canine von Willebrand Factor ELISA | |
| KLN0323 Canine Thrombin-Antithrombin Complex ELISA | |
| KLN0274 Canine Coagulation Factor 2 ELISA | |
| KLN0275 Canine Coagulation Factor 5 ELISA | |
| KLN0276 Canine Coagulation Factor 7 ELISA | |
| KLN0277 Canine Coagulation Factor 9 ELISA | |
| KLN0278 Canine Coagulation Factor 10 ELISA | |
| KLN0279 Canine Coagulation Factor 11 ELISA | |
| KLN0280 Canine Coagulation Factor 12 ELISA | |
| KLN0281 Canine Coagulation Factor 13 ELISA | |
| KLN0282 Canine Coagulation Factor 14 ELISA | |
| Horse | KLV0038 Horse Thrombin Activatable Fibrinolysis Inhibitor ELISA |
| KLV0039 Horse Plasminogen Activator (Urokinase) ELISA | |
| KLV0041 Horse Plasminogen Activator Inhibitor 2 ELISA | |
| KLV0043 Horse Tissue Factor Pathway Inhibitor ELISA | |
| Guinea Pig | KLY0004 Guinea Pig Anti-Thrombin ELISA |
| Monkey | KLW0136 Monkey Tissue Factor Pathway Inhibitor ELISA |