ELISA Tips

Causes Of High Background In ELISA Tests and How to Solve Them ELISA tests are widely used in medical diagnostics, research, and Drug Development and various fields of biology. However, since they are often run manually, there are chances of human error that may cause high background and therefore incorrect results and quantification of analyte. […]

How To: Correctly Reconstitute Lyophilised Reagents How To Correctly Reconstitute Lyophilized Reagents for Use in ELISA Lyophilized reagents are a common form of storage for various substances in research labs. Most of Krishgen’s KRIBIOLISA immunoassays and several GENLISA assays come with lyophilised standards for better stability. Whether you’re an experienced research scientist or just starting […]

ELISA Sample Preparation FAQs Krishgen’s ELISA are available under two product types: GENLISATM branded products are ELISA for cytokine and biomarker testing in various species including humans, rat, mouse and other species. These ELISA have been validated for use with serum, plasma and cell culture supernatant samples. KRIBIOLISATM branded products are specialised for biopharma related […]

GraphPad Prism: Tips and tricks for better analysis and reports GraphPad Prism remains a preferred app for scientists for data analysis, especially when running ELISA and calculating concentrations. Whether you’re a seasoned user or just starting out on the software, there are a number of advanced tips and tricks that can help you get more […]

Best Practices for Serum and Plasma Extraction for Using with ELISA The accuracy and reliability of results in an ELISA depend heavily on the quality of the extracted serum or plasma. In this blog post, we will discuss the best practices for serum and plasma extraction for ELISA to ensure optimal sample quality and assay […]

NIBSC Standards and their relevance in ELISA validation What is NIBSC Validation and how is it relevant? One of the issues that any scientist – especially in biotech and pharma – may face is that the quantification of their target product is subjective to the ELISA or assay they use, and the calibration standards included […]

Types of ELISA: Sandwich, Competitive, Direct and Indirect There are many ELISA tests for particular molecules that use the matching antibodies. ELISA tests are broken into several types of tests based on how the analytes and antibodies are bonded and used. The major types are described here. Direct ELISA The steps of direct ELISA follows […]