Description
Background:
Ertumaxomab is an intact bispecific antibody targeting HER2/neu and CD3 with preferential binding to activating Fcgamma type I/III-receptors, resulting in the formation of a tri-cell complex among tumour cell, T cell and accessory cell. Recently, the antibody demonstrated antitumour efficacy against HER2/neu low-expressing tumours resistant to trastuzumab.
Intended Use:
For Estimation of Ertumaxomab (REXOMUN) in human serum and plasma. Please note the kit has not been optimized to be used for any other animal species. Should you require a specific sera tested, please connect with us at sales@krishgen.com for optimization.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Ertumaxomab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Ertumaxomab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Ertumaxomab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Ertumaxomab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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