Description
Introduction:
Recombinant expression by the hybridoma cell line SP2/0 is a relatively simple and cost effective method for production of monoclonal antibodies intended for use as therapeutic agents in humans.
Intended Use:
This generic kit is intended in determining the presence of SP2/0 Host Cell Proteins contamination in various products that are manufactured through recombinant expression in SP2/0 cells. The kit has been validated successfully for testing of in process and final product HCPs in variety of products regardless of growth and purification process.
Principle:
This assay is based on the Sandwich ELISA procedure. Microwells are coated with anti- SP2/0 antibody. Addition of Anti- SP2/0:HRP Conjugate and standards or samples containing SP2/0 HCPs will form a immune complex with the coated anti- SP2/0 antibody in microwell. A washing cycle will remove free Anti- SP2/0:HRP Conjugate from the reaction well. Addition of TMB Substrate will produce blue color in microwells conataining immune complex. Addition of stop solution will terminate the enzymatic reaction and blue color will be converted to yellow color which is directly proportional to the concentration of SP2/0 HCPs present and absorbance value is measured at 450nm.
If you have published a paper by using any of our ELISA since 01/01/2023, kindly fill out the “Krishgen Publication Reward Application Form” with complete information and send it by at email: info@krishgen.com, with the subject “Krishgen Publication Reward”. We will get back to you with the Amazon / Krishgen Credit Reward after we confirm it ASAP!